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osubip cas9 nost  (Addgene inc)


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    Structured Review

    Addgene inc osubip cas9 nost
    Osubip Cas9 Nost, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbi+mcs+egfp/pBI-MCS-EGFP+(Plasmid+%2316542)/pm41662274-60-44-45
    Average 93 stars, based on 24 article reviews
    osubip cas9 nost - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Bloom helicase mediates formation of large single–stranded DNA loops during DNA end processing
    Article Snippet: U2OS cells were grown in Dulbecco’s Modified Eagle Medium medium (Thermo Fisher, Cat. No.: 10569010) supplemented with 10% V/V Fetal Bovine Serum (Fisher Scientific, Cat. No.: CorningTM 35011CV) and 1% V/V penicillin-streptomycin antibiotic (Thermo Fisher, Cat. No.: 15140122). .. pBI–MCS–EGFP (a generous gift from Bert Vogelstein (Addgene, plasmid #16542) containing WT-BLM, BLM Δ1–641 and BLM Δ1291–1330 were used to transfect the U2OS cells in a 6 well using Lipofectamine 3000 Transfection Reagent (Invitrogen, Cat. No.: L3000015). .. In total, 25 nM of siRNA mix containing siBLM (5′–GAA UCU CAA UGU ACA UAG AUU UU; Dharmacon), siEXO1 (5′–UGC CUU UGC UAA UCC AAU CCC ACG C; Invitrogen) or the siScramble control Dharmacon ON-TARGET plus Non-Targeting Control siRNA 1 Cat. No.: D–001810–01–05) were added to the cells 24 h after plasmid transfection, using RNAiMax regent LipofectamineTM RNAiMAX Transfection Reagent (Invitrogen, Cat. No.: 13778075).

    Article Title: Acquired SETD2 mutation and impaired CREB1 activation confer cisplatin resistance in metastatic non-small cell lung cancer
    Article Snippet: Cell cytotoxicity and apoptosis The CCK-8 kit (VitaScientific, MD, USA) was used to determine cell viability. .. A549, H460, H157, and H358 cells were transfected with 1 μg of pBI-MCS-EGFP (Addgene) or mutant SETD2 (mtSETD2)-GFP plasmid for overexpressing stable cell lines. ..

    Transfection:

    Article Title: Bloom helicase mediates formation of large single–stranded DNA loops during DNA end processing
    Article Snippet: U2OS cells were grown in Dulbecco’s Modified Eagle Medium medium (Thermo Fisher, Cat. No.: 10569010) supplemented with 10% V/V Fetal Bovine Serum (Fisher Scientific, Cat. No.: CorningTM 35011CV) and 1% V/V penicillin-streptomycin antibiotic (Thermo Fisher, Cat. No.: 15140122). .. pBI–MCS–EGFP (a generous gift from Bert Vogelstein (Addgene, plasmid #16542) containing WT-BLM, BLM Δ1–641 and BLM Δ1291–1330 were used to transfect the U2OS cells in a 6 well using Lipofectamine 3000 Transfection Reagent (Invitrogen, Cat. No.: L3000015). .. In total, 25 nM of siRNA mix containing siBLM (5′–GAA UCU CAA UGU ACA UAG AUU UU; Dharmacon), siEXO1 (5′–UGC CUU UGC UAA UCC AAU CCC ACG C; Invitrogen) or the siScramble control Dharmacon ON-TARGET plus Non-Targeting Control siRNA 1 Cat. No.: D–001810–01–05) were added to the cells 24 h after plasmid transfection, using RNAiMax regent LipofectamineTM RNAiMAX Transfection Reagent (Invitrogen, Cat. No.: 13778075).

    Article Title: Acquired SETD2 mutation and impaired CREB1 activation confer cisplatin resistance in metastatic non-small cell lung cancer
    Article Snippet: Cell cytotoxicity and apoptosis The CCK-8 kit (VitaScientific, MD, USA) was used to determine cell viability. .. A549, H460, H157, and H358 cells were transfected with 1 μg of pBI-MCS-EGFP (Addgene) or mutant SETD2 (mtSETD2)-GFP plasmid for overexpressing stable cell lines. ..

    Recombinant:

    Article Title: Augmenter of liver regeneration regulates cellular iron homeostasis by modulating mitochondrial transport of ATP-binding cassette B8
    Article Snippet: Recombinant DNA reagent , Mouse Tfrc promoter luciferase construct , This manuscript , N/A , . .. Recombinant DNA reagent , pBI-MCS-EGFP , Addgene , 16542 , . .. Recombinant DNA reagent , pLenti CMV rtTA3 Blast (w756-1) , Addgene , 26429 , .

    Generated:

    Article Title: Adaptation by stochastic tuning of gene expression in mammalian cells
    Article Snippet: .. We first generated a minimal expression reporter by replacing the eGFP cassette in pBI-MCS-eGFP (Addgene #16542) with an mDHFR(L22R)-mRuby fusion. ..

    Article Title: Essential roles of Tbr1 in the formation and maintenance of the orientation-selective J-RGCs and a group of OFF-sustained RGCs in mouse
    Article Snippet: .. TetO-Tbr1:eGFP mice (described in Fig. S7 ) were generated by inserting a Tbr1 cDNA fragment into the PvuII site of PBI-MCS-eGFP (Addgene, #16542). ..

    Expressing:

    Article Title: Adaptation by stochastic tuning of gene expression in mammalian cells
    Article Snippet: .. We first generated a minimal expression reporter by replacing the eGFP cassette in pBI-MCS-eGFP (Addgene #16542) with an mDHFR(L22R)-mRuby fusion. ..

    Mutagenesis:

    Article Title: Acquired SETD2 mutation and impaired CREB1 activation confer cisplatin resistance in metastatic non-small cell lung cancer
    Article Snippet: Cell cytotoxicity and apoptosis The CCK-8 kit (VitaScientific, MD, USA) was used to determine cell viability. .. A549, H460, H157, and H358 cells were transfected with 1 μg of pBI-MCS-EGFP (Addgene) or mutant SETD2 (mtSETD2)-GFP plasmid for overexpressing stable cell lines. ..

    Stable Transfection:

    Article Title: Acquired SETD2 mutation and impaired CREB1 activation confer cisplatin resistance in metastatic non-small cell lung cancer
    Article Snippet: Cell cytotoxicity and apoptosis The CCK-8 kit (VitaScientific, MD, USA) was used to determine cell viability. .. A549, H460, H157, and H358 cells were transfected with 1 μg of pBI-MCS-EGFP (Addgene) or mutant SETD2 (mtSETD2)-GFP plasmid for overexpressing stable cell lines. ..



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